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All procedures used in animal experiments were approved by the Animal Ethics Committee of the institution

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Cytotoxicity The cytotoxicity of conventional and PEGylated liposomes encapsulated with stigmasterol and its esters with myristic and oleic acids before and after their gastrointestinal digestion (both gastric and intestine stages) was assessed using human normal small intestinal HIEC-6 (ATCC CRL-3266) and colon mucosa CCD 841CoN (ATCC CRL-179) cells obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA)

Treatment had no effect on Iba1 + microglia or regional brain weights and did not affect motor development or the expression of genes related to IGF-1 signaling

In their research, they analyzed 40 patients out of whom 24 received a total of 12 g/day NAC intravenously divided into four doses and 16 patients received standard medical therapy only (including penicillin G)